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Journal of Clinical Microbiology, September 2004, p. 4032-4039, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4032-4039.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Characterizations of Adenovirus Type 41 Isolates from Children with Acute Gastroenteritis in Japan, Vietnam, and Korea

Lei Li,1 Hideaki Shimizu,2 Lan Thi Phuong Doan,1 Phan Gia Tung,1 Shoko Okitsu,1 Osamu Nishio,3 Eiko Suzuki,1 Jeong Kee Seo,4 Kyo Sun Kim,5 Werner E. G. Müller,6 and Hiroshi Ushijima1*

Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo,1 Infectious Surveillance Center, National Institute of Infectious Diseases, Tokyo,3 Kawasaki City Institute of Public Health, Kawasaki City, Japan,2 Seoul National University Children's Hospital,4 Kon-Kuk University Chung Ju Hospital, Seoul, Korea,5 Institut für Physiologische Chemie, Mainz University, Mainz, Germany6

Received 29 February 2004/ Returned for modification 24 April 2004/ Accepted 23 May 2004

Genetic and antigenic characterizations of 70 strains of adenovirus type 41 (Ad41), isolated between 1998 and 2001 from children in Japan, Vietnam, and Korea, were done by DNA restriction enzyme (RE) analysis, sequencing analysis, and monoclonal antibody (MAb)-based enzyme-linked immunosorbent assay (ELISA). Eight genome types were observed in the present study, among which D25, D26, D27, and D28 were novel genome types. These eight genome types were divided into two genome-type clusters (GTCs) based on phylogenetic analysis of the hypervariable regions (HVRs) of the hexon. GTC1 includes D1, D25, D26, D27, and D28, and the GTC2 contains D4, D12, and D22. The amino acid homologies among the members within a GTC were 97 to 100%, whereas between the members of different GTCs the homologies were 92 to 94%. The specificity of the GTC classification was confirmed by ELISA with MAb 1F, which was selected by the Ad41 prototype Tak strain. It was found that only the isolates of GTC1 but not of GTC2 reacted with MAb 1F. These results suggest that Ad41 isolates from the three countries should be classified into two subtypes. The accumulation of amino acid mutations located in HVRs of hexon are indicative for the classification of Ad41 subtype.

* Corresponding author. Mailing address: Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-Ku, Tokyo 113-0033, Japan. Phone: (81) 3-5841-3629. Fax: (81) 3-5841-3629. E-mail: ushijima{at}m.u-tokyo.ac.jp.

Journal of Clinical Microbiology, September 2004, p. 4032-4039, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4032-4039.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhang, Q., Su, X., Gong, S., Zeng, Q., Zhu, B., Wu, Z., Peng, T., Zhang, C., Zhou, R. (2006). Comparative genomic analysis of two strains of human adenovirus type 3 isolated from children with acute respiratory infection in southern China. J. Gen. Virol. 87: 1531-1541 [Abstract] [Full Text]  


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