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Journal of Clinical Microbiology, September 2004, p. 4137-4140, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4137-4140.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification of the Emerging Pathogen Vibrio vulnificus Biotype 3 by Commercially Available Phenotypic Methods

Raul Colodner,1* Raul Raz,2 Irit Meir,3 Tsilia Lazarovich,4 Larisa Lerner,5 June Kopelowitz,5 Yoram Keness,1 Waheeb Sakran,6 Shifra Ken-Dror,7 and Naiel Bisharat8

Clinical Microbiology Laboratory,1 Infectious Diseases Unit,2 Pediatric Department B, Ha'Emek Medical Center, Afula,6 Maccabi Health Services Laboratory,3 Klalit Health Services General Laboratory, Haifa,7 Clinical Microbiology Laboratory, Assaf Harofeh Medical Center, Zerifin,4 Israel Ministry of Health Central Laboratories, Jerusalem, Israel,5 Nuffield Department of Clinical Laboratory Sciences, John Radcliffe Hospital, University of Oxford, Oxford, United Kingdom8

Received 3 February 2004/ Returned for modification 10 March 2004/ Accepted 2 June 2004

Identification of the emerging pathogen Vibrio vulnificus biotype 3 has become a challenge for clinical laboratories in the last few years. In this study, the abilities of five commercial systems to identify this new species have been evaluated for the first time, using a unique collection of strains. Fifty-one well-documented wild strains of V. vulnificus biotype 3 were processed using API 20 NE, GNI+ Vitek 1 cards, ID-GNB Vitek 2 cards, Neg Combo 20 Microscan panels, and NMIC/ID-5 BD Phoenix panels. The numbers of strains identified as V. vulnificus by ID-GNB, NMIC/ID-5, and GNI+ were 50 (98.0%), 46 (90.2%), and 7 (13.7%), respectively. Neg Combo 20 Microscan panels and API 20 NE were unable to identify any of the strains of this emerging pathogen to the species level and mostly misidentifies them as other species of the Vibrionaceae family. Data on the phenotypic pattern of V. vulnificus biotype 3 when processed in all five systems as presented here could help clinical laboratories in identifying this new pathogen.

* Corresponding author. Mailing address: Clinical Microbiology Laboratory, Ha'Emek Medical Center, Afula 18101, Israel. Phone: 972 4649 4480. Fax: 972 4649 5428. E-mail: colodner_ra{at}clalit.org.il.

Journal of Clinical Microbiology, September 2004, p. 4137-4140, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4137-4140.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sanjuan, E., Fouz, B., Oliver, J. D., Amaro, C. (2009). Evaluation of Genotypic and Phenotypic Methods To Distinguish Clinical from Environmental Vibrio vulnificus Strains. Appl. Environ. Microbiol. 75: 1604-1613 [Abstract] [Full Text]  
  • Muldrew, K. L., Miller, R. R., Kressin, M., Tang, Y.-W., Stratton, C. (2007). Necrotizing Fasciitis from Vibrio vulnificus in a Patient with Undiagnosed Hepatitis and Cirrhosis. J. Clin. Microbiol. 45: 1058-1062 [Abstract] [Full Text]  
  • O'Hara, C. M. (2006). Evaluation of the Phoenix 100 ID/AST System and NID Panel for Identification of Enterobacteriaceae, Vibrionaceae, and Commonly Isolated Nonenteric Gram-Negative Bacilli.. J. Clin. Microbiol. 44: 928-933 [Abstract] [Full Text]  


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