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Journal of Clinical Microbiology, September 2004, p. 4199-4203, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4199-4203.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Correlation of Cerebrospinal Fluid (CSF) Cell Counts and Elevated CSF Protein Levels with Enterovirus Reverse Transcription-PCR Results in Pediatric and Adult Patients

William S. Mulford,1 Richard S. Buller,2 Max Q. Arens,2* and Gregory A. Storch2,3

St. Louis Children's Hospital,1 Departments of Pediatrics,2 Medicine, and Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri3

Received 30 March 2004/ Returned for modification 15 April 2004/ Accepted 28 May 2004

During the 2001, 2002, and 2003 enterovirus seasons, we investigated the correlations between cerebrospinal fluid (CSF) nucleated cell counts and elevated CSF protein levels and the detection of enteroviral RNA by reverse transcription (RT)-PCR. Our objective was to determine if pleocytosis and/or elevated protein levels were predictive of positive RT-PCR results for enterovirus. We were also interested in determining if the presence of West Nile virus during the 2002 enteroviral season contributed to a change in these correlations. We found that in the group of patients aged >2 months, the absence of pleocytosis was highly predictive of a negative RT-PCR result. Elevated CSF protein level was not a good predictor of RT-PCR positivity for enterovirus and did not add to the diagnostic sensitivity or specificity of pleocytosis.

* Corresponding author. Mailing address: Department of Pediatrics, Box 8116, Washington University School of Medicine, One Children's Pl., St. Louis, MO 63110. Phone: (314) 454-8035. Fax: (314) 454-8020. E-mail: arens{at}kids.wustl.edu.

Journal of Clinical Microbiology, September 2004, p. 4199-4203, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4199-4203.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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