This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lin, S.-Y. G.
Right arrow Articles by Desmond, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lin, S.-Y. G.
Right arrow Articles by Desmond, E.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2004, p. 4204-4208, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4204-4208.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Rapid Detection of Isoniazid and Rifampin Resistance Mutations in Mycobacterium tuberculosis Complex from Cultures or Smear-Positive Sputa by Use of Molecular Beacons

S.-Y. Grace Lin, William Probert, Melanie Lo, and Edward Desmond*

Microbial Diseases Laboratory, California Department of Health Services, Richmond, California 94804

Received 29 October 2003/ Returned for modification 20 February 2004/ Accepted 12 May 2004

The slow-growing nature of Mycobacterium tuberculosis complex hinders the improvement of turnaround time for phenotypic drug susceptibility testing. We designed a set of molecular beacons for the detection of isoniazid and rifampin resistance mutations in M. tuberculosis complex organisms from cultures or from N-acetyl-L-cysteine-NaOH-treated, smear-positive specimens. The performance of the molecular beacons was characterized by studying a total of 196 clinical isolates (127 drug-resistant isolates and 69 drug-susceptible isolates). For detection of isoniazid resistance, the sensitivity and specificity of the assay were 82.7 and 100%, and the positive predictive value (PPV) and negative predictive value (NPV) at a resistance prevalence of 10% were 100 and 98.11%, respectively. For detection of rifampin resistance, the sensitivity and specificity of the assay were 97.5 and 100%, and the PPV and NPV at a resistance prevalence of 2.0% were 100 and 99.95%, respectively.

* Corresponding author. Mailing address: Microbial Diseases Laboratory, CDHS, 850 Marina Bay Pkwy., Richmond, CA 94804. Phone: (510) 412-3781. Fax: (510) 412-3706. E-mail: edesmond{at}dhs.ca.gov.

Journal of Clinical Microbiology, September 2004, p. 4204-4208, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4204-4208.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lin, S.-Y. G., Desmond, E., Bonato, D., Gross, W., Siddiqi, S. (2009). Multicenter Evaluation of Bactec MGIT 960 System for Second-Line Drug Susceptibility Testing of Mycobacterium tuberculosis Complex. J. Clin. Microbiol. 47: 3630-3634 [Abstract] [Full Text]  
  • Bahrmand, A. R., Titov, L. P., Tasbiti, A. H., Yari, S., Graviss, E. A. (2009). High-Level Rifampin Resistance Correlates with Multiple Mutations in the rpoB Gene of Pulmonary Tuberculosis Isolates from the Afghanistan Border of Iran. J. Clin. Microbiol. 47: 2744-2750 [Abstract] [Full Text]  
  • Richardson, E. T., Samson, D., Banaei, N. (2009). Rapid Identification of Mycobacterium tuberculosis and Nontuberculous Mycobacteria by Multiplex, Real-Time PCR. J. Clin. Microbiol. 47: 1497-1502 [Abstract] [Full Text]  
  • Evans, J. T., Parveen, A., Smith, G. E., Xu, L., Chan, E. W. C., Chan, R. C. Y., Hawkey, P. M. (2009). Application of denaturing HPLC to rapidly identify rifampicin-resistant Mycobacterium tuberculosis in low- and high-prevalence areas. J Antimicrob Chemother 63: 295-301 [Abstract] [Full Text]  
  • Yesilkaya, H., Meacci, F., Niemann, S., Hillemann, D., Rusch-Gerdes, S., LONG DRUG Study Group, , Barer, M. R., Andrew, P. W., Oggioni, M. R. (2006). Evaluation of Molecular-Beacon, TaqMan, and Fluorescence Resonance Energy Transfer Probes for Detection of Antibiotic Resistance-Conferring Single Nucleotide Polymorphisms in Mixed Mycobacterium tuberculosis DNA Extracts.. J. Clin. Microbiol. 44: 3826-3829 [Abstract] [Full Text]  
  • Balashov, S. V., Park, S., Perlin, D. S. (2006). Assessing Resistance to the Echinocandin Antifungal Drug Caspofungin in Candida albicans by Profiling Mutations in FKS1.. Antimicrob. Agents Chemother. 50: 2058-2063 [Abstract] [Full Text]  
  • Espy, M. J., Uhl, J. R., Sloan, L. M., Buckwalter, S. P., Jones, M. F., Vetter, E. A., Yao, J. D. C., Wengenack, N. L., Rosenblatt, J. E., Cockerill, F. R. III, Smith, T. F. (2006). Real-Time PCR in Clinical Microbiology: Applications for Routine Laboratory Testing. Clin. Microbiol. Rev. 19: 165-256 [Abstract] [Full Text]  
  • Nahid, P., Pai, M., Hopewell, P. C. (2006). Advances in the diagnosis and treatment of tuberculosis.. Proc Am Thorac Soc 3: 103-110 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»