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Journal of Clinical Microbiology, September 2004, p. 4253-4260, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4253-4260.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification and Typing of Malassezia Species by Amplified Fragment Length Polymorphism and Sequence Analyses of the Internal Transcribed Spacer and Large-Subunit Regions of Ribosomal DNA

Aditya K. Gupta,1,2 Teun Boekhout,3 Bart Theelen,3 Richard Summerbell,3 and Roma Batra2*

Division of Dermatology, Department of Medicine, Sunnybrook and Women's College Health Science Center, the University of Toronto, Toronto,1 Mediprobe Laboratories, London, Ontario, Canada,2 Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands3

Received 8 January 2004/ Returned for modification 3 March 2004/ Accepted 28 March 2004

Malassezia yeasts are associated with several dermatological disorders. The conventional identification of Malassezia species by phenotypic methods is complicated and time-consuming, and the results based on culture methods are difficult to interpret. A comparative molecular approach based on the use of three molecular techniques, namely, amplified fragment length polymorphism (AFLP) analysis, sequencing of the internal transcribed spacer, and sequencing of the D1 and D2 domains of the large-subunit ribosomal DNA region, was applied for the identification of Malassezia species. All species could be correctly identified by means of these methods. The results of AFLP analysis and sequencing were in complete agreement with each other. However, some discrepancies were noted when the molecular methods were compared with the phenotypic method of identification. Specific genotypes were distinguished within a collection of Malassezia furfur isolates from Canadian sources. AFLP analysis revealed significant geographical differences between the North American and European M. furfur strains.

* Corresponding author. Mailing address: Mediprobe Laboratories, 645 Windermere Rd., London, Ontario, Canada. Phone: (519) 657-4222. Fax: (519) 657-4233. E-mail: agupta{at}mediproberesearch.com.

Journal of Clinical Microbiology, September 2004, p. 4253-4260, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4253-4260.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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