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Journal of Clinical Microbiology, September 2004, p. 4310-4312, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4310-4312.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Fosfomycin and Rifampin Disk Diffusion Tests for Detection of Escherichia coli Mutator Strains

Juan-Carlos Galán,1* Marta Tato,1 María-Rosario Baquero,1 Carmen Turrientes,1 Fernando Baquero,1 and José-Luis Martinez2

Servicio de Microbiología, Hospital Universitario Ramón y Cajal,1 Centro Nacional de Biotecnología, Madrid, Spain2

Received 24 February 2004/ Returned for modification 13 April 2004/ Accepted 13 May 2004

A simple method, using commercial disks to detect Escherichia coli mutator strains, is proposed. The breakpoint for detecting strains with a mutation frequency ≥5 x 10–7 was established at ≥70 and ≥20 colonies in the inhibition zone of fosfomycin and rifampin disks, respectively, after seeding 100 µl of an overnight culture. Strains with <30 and <10 colonies in fosfomycin and rifampin inhibition zones are presumptively non-mutators.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Ctra. de Colmenar, Km 9,1, 28034 Madrid, Spain. Phone: 34-91-336 8330. Fax: 34-91-336 8809. E-mail: jgalanm.hrc{at}salud.madrid.org.

Journal of Clinical Microbiology, September 2004, p. 4310-4312, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4310-4312.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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