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Journal of Clinical Microbiology, September 2004, p. 4327-4328, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4327-4328.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of Conventional Castaneda and Lysis Centrifugation Blood Culture Techniques for Diagnosis of Human Brucellosis

Basappa G. Mantur* and Smita S. Mangalgi

Department of Microbiology, B.L.D.E.A's Shri B. M. Patil Medical College, Bijapur, Karnataka, India

Received 7 March 2004/ Returned for modification 12 May 2004/ Accepted 28 May 2004

We investigated the role of the lysis centrifugation blood culture technique over the conventional Castaneda technique for the diagnosis of human brucellosis. The lysis centrifugation technique has been found to be more sensitive in both acute (20% higher sensitivity; P < 0.00001) and chronic (40% higher sensitivity; P = 0.087) forms of brucellosis. The major advantage of lysis centrifugation was in the mean detection time, which was only 2.4 days in acute and 2.7 days in chronic cases, with 103 out of 110 (93.6%) and 17 out of 20 (85%) cultures from acute and chronic brucellosis, respectively, detected before the conventional culture was positive. Our results confirmed the potential usefulness of the lysis technique in diagnosis and institution of appropriate antibiotic therapy.

* Corresponding author. Mailing address: Department of Microbiology, B.L.D.E.A's Shri B. M. Patil Medical College, Bijapur 586 103, Karnataka, India. Phone: (91) 8352-262770, ext. 2227. Fax: (91) 8352-263019. E-mail: drbgmantur{at}rediffmail.com.

Journal of Clinical Microbiology, September 2004, p. 4327-4328, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4327-4328.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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