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Journal of Clinical Microbiology, September 2004, p. 4332-4334, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4332-4334.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Multicenter Validation of the cppB Gene as a PCR Target for Detection of Neisseria gonorrhoeae

S. M. Bruisten,1* G. T. Noordhoek,2 A. J. C. van den Brule,3 B. Duim,4 C. H. E. Boel,3 K. El-Faouzi,1 R. du Maine,4 S. Mulder,2 D. Luijt,5 and J. Schirm5

Municipal Health Service, GG&GD,1 Department of Medical Microbiology, AMC, Amsterdam,4 Public Health Laboratory Friesland, LVF, Leeuwarden,2 Laboratory for Pathology and Microbiology, PAMM, Veldhoven,3 Regional Public Health Laboratory, SLGD, Groningen, The Netherlands5

Received 19 February 2004/ Returned for modification 29 March 2004/ Accepted 18 May 2004

The cppB gene is often used as a target for detection of Neisseria gonorrhoeae by PCR. Using a coded panel of 500 DNA samples, we determined that the cppB gene is missing in 5.8% of N. gonorrhoeae strains, and therefore we consider the cppB gene to be an unsuitable target.

* Corresponding author. Mailing address: GG&GD Municipal Health Service, Public Health Laboratory, Nieuwe Achtergracht 100, 1018 WT Amsterdam, The Netherlands. Phone: 31-20-555.5376. Fax: 31-20-555.5629. E-mail: sbruisten{at}gggd.amsterdam.nl.

Journal of Clinical Microbiology, September 2004, p. 4332-4334, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4332-4334.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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