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Journal of Clinical Microbiology, September 2004, p. 4355-4357, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4355-4357.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Pneumolysin Is a Key Factor in Misidentification of Macrolide-Resistant Streptococcus pneumoniae and Is a Putative Virulence Factor of S. mitis and Other Streptococci

Chris Neeleman,1 Corné H. W. Klaassen,2 Debbie M. Klomberg,2 Hanneke A. de Valk,2 and Johan W. Mouton2*

Department of Intensive Care Medicine, University Hospital St. Radboud,1 Department of Medical Microbiology and Infectious Diseases, Canisius/Wilhelmina Hospital, Nijmegen, The Netherlands2

Received 18 February 2004/ Returned for modification 6 April 2004/ Accepted 26 May 2004

We evaluated the applicability of ply PCR for confirmation of the identification of Streptococcus pneumoniae. lytA PCR, 16S rRNA sequencing, and amplified-fragment length polymorphism were used as reference methods. In contrast to the lytA gene, the ply gene proved to be not specific for S. pneumoniae. The presence of the ply gene in other streptococci, in particular Streptococcus mitis, suggests that pneumolysin plays a pathogenic role.

* Corresponding author. Mailing address: Department of Medical Microbiology and Infectious Diseases, Canisius Wilhelmina Ziekenhuis Nijmegen, Weg door Jonkerbos 100, 6532 sz Nijmegen, The Netherlands. Phone: 00-31-(0)24-3657514. Fax: 00-31-(0)24-3657516. E-mail: Mouton{at}cwz.nl.

Journal of Clinical Microbiology, September 2004, p. 4355-4357, Vol. 42, No. 9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4355-4357.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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