This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yun, Y.-J.
Right arrow Articles by Kook, Y.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yun, Y.-J.
Right arrow Articles by Kook, Y.-H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2005, p. 174-178, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.174-178.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Detection and Identification of Mycobacterium tuberculosis in Joint Biopsy Specimens by rpoB PCR Cloning and Sequencing

Yeo-Jun Yun,1 Keun-Hwa Lee,1 Lin Haihua,1 Yoon-Jong Ryu,1 Bum-Joon Kim,1 Young-Ho Lee,2 Goo-Hyun Baek,3 Hee-Joong Kim,3 Moon-Sang Chung,3 Myung-Chul Lee,3 Sang-Hoon Lee,3 In-Ho Choi,3 Tae-Jun Cho,3 Bong-Soon Chang,3 and Yoon-Hoh Kook1*

Department of Microbiology and Cancer Research Institute, Institute of Endemic Diseases, SNUMRC, Seoul National University College of Medicine, and Clinical Research Institute, Seoul National University Hospital,1 Department of Orthopedic Surgery, Soonchunhyang University Hospital,2 Department of Orthopedic Surgery, Seoul National University College of Medicine, Seoul, Korea3

Received 16 March 2004/ Returned for modification 8 July 2004/ Accepted 26 August 2004

Osteoarticular tuberculosis (OAT) is an extrapulmonary tuberculosis and accounts for 1 to 3% of all tuberculosis cases. We used an rpoB PCR-plasmid TA cloning-sequencing method to detect and identify tubercle bacilli in surgical specimens from patients suspected of having OAT. By comparing the similarities of the rpoB sequences determined with those in GenBank, Mycobacterium tuberculosis was detected in 23 of 43 samples. Three of the 23 positive samples had mutations at codon 531, which are commonly observed in rifampin-resistant M. tuberculosis strains. Our results suggest that the rpoB PCR-TA cloning-sequencing method developed, which detects M. tuberculosis and which simultaneously determines its rifampin susceptibility, can also be used efficiently for the diagnosis of OAT.

* Corresponding author. Mailing address: Department of Microbiology, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Korea. Phone: (82) 2-740-8306. Fax: (82) 2-743-0881. E-mail: yhkook{at}plaza.snu.ac.kr.

Journal of Clinical Microbiology, January 2005, p. 174-178, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.174-178.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bahrmand, A. R., Titov, L. P., Tasbiti, A. H., Yari, S., Graviss, E. A. (2009). High-Level Rifampin Resistance Correlates with Multiple Mutations in the rpoB Gene of Pulmonary Tuberculosis Isolates from the Afghanistan Border of Iran. J. Clin. Microbiol. 47: 2744-2750 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»