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Journal of Clinical Microbiology, January 2005, p. 314-320, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.314-320.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of Variable-Number Tandem-Repeat Typing To Differentiate Mycobacterium tuberculosis Beijing Family Isolates from Hong Kong and Comparison with IS6110 Restriction Fragment Length Polymorphism Typing and Spoligotyping

Kristin Kremer,1 Betty Kam Yan Au,1 Peter Chi Wai Yip,2 Robin Skuce,3 Philip Supply,4 Kai Man Kam,2 and Dick van Soolingen1*

Mycobacteria Reference Unit, Diagnostic Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, Bilthoven, The Netherlands,1 Tuberculosis Reference Laboratory, Public Health Laboratory Center, Department of Health, Hong Kong,2 Veterinary Sciences Division, Department of Agriculture and Rural Development, Belfast, Northern Ireland,3 Laboratoire des Mécanismes Moléculaires de la Pathogénèse Bactérienne, INSERM U629, Institut Pasteur de Lille, Lille, France4

Received 24 June 2004/ Returned for modification 14 September 2004/ Accepted 21 September 2004

The Mycobacterium tuberculosis Beijing family isolates may cause more than a quarter of all tuberculosis cases worldwide, are emerging in some areas, and are often associated with drug resistance. Early recognition of transmission of this genotype is therefore important. To evaluate the usefulness of variable-number tandem-repeat (VNTR) typing to discriminate and recognize strains of the Beijing family, M. tuberculosis isolates from Hong Kong were subjected to VNTR analysis, spoligotyping, and IS6110 restriction fragment length polymorphism (RFLP) typing. The allelic diversity of the 14 VNTR loci included in the analysis varied from 0 to 0.618 among Beijing strains. The discriminatory power of VNTR analysis was slightly lower than that of IS6110 RFLP. Our analysis shows that VNTR typing, which has many practical advantages over RFLP typing, can be used for epidemiological studies of Beijing strains. However, VNTR-defined clusters should be subtyped with IS6110 RFLP for maximal resolution.

* Corresponding author. Mailing address: Head Mycobacteria Reference Unit, Diagnostic Laboratory for infectious Diseases and Perinatal Screening (LIS, pb22), P.O. Box 1, 3720 BA Bilthoven, The Netherlands. Phone: 31 30 2742363. Fax: 31 30 2744418. E-mail: D.van.soolingen{at}rivm.nl.

Journal of Clinical Microbiology, January 2005, p. 314-320, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.314-320.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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