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Journal of Clinical Microbiology, January 2005, p. 439-441, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.439-441.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of an Accelerated Protocol for Detection of Extended-Spectrum ß-Lactamase-Producing Gram-Negative Bacilli from Positive Blood Cultures

Shiri Navon-Venezia,1 Azita Leavitt,1 Ronen Ben-Ami,2 Yuval Aharoni,3 Mitchell J. Schwaber,1,4 David Schwartz,3 and Yehuda Carmeli1,2,4*

Laboratories for Molecular Epidemiology,1 Microbiology,3 Divisions of Epidemiology,4 Infectious Diseases, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel2

Received 2 August 2004/ Returned for modification 30 August 2004/ Accepted 13 September 2004

We evaluated a protocol for the accelerated detection of extended-spectrum ß-lactamases (ESBLs) in gram-negative bloodstream pathogens. Two hundred eighty-three blood culture bottles were subjected to direct ESBL testing by inoculating samples directly from blood culture bottles onto agar plates containing cefotaxime and ceftazidime disks, with and without clavulanate. Standard ESBL testing in accordance with the NCCLS guidelines after subculturing on agar plates was performed in parallel. Results of the direct ESBL testing were reported 2.3 days sooner and were comparable to those of the standard NCCLS method with sensitivity, specificity, and positive and negative predictive values of 100, 98, 94, and 100%, respectively.

* Corresponding author. Mailing address: Molecular Epidemiology Laboratory, Tel-Aviv Sourasky Medical Center, 6 Weizman St., Tel-Aviv 64239, Israel. Phone: 972-3-692 5644. Fax: 972-3-697 4623. E-mail: shiri_nv{at}tasmc.health.gov.il.

Journal of Clinical Microbiology, January 2005, p. 439-441, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.439-441.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Anderson, D. J., Engemann, J. J., Harrell, L. J., Carmeli, Y., Reller, L. B., Kaye, K. S. (2006). Predictors of Mortality in Patients with Bloodstream Infection Due to Ceftazidime-Resistant Klebsiella pneumoniae.. Antimicrob. Agents Chemother. 50: 1715-1720 [Abstract] [Full Text]  


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