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Journal of Clinical Microbiology, January 2005, p. 448-451, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.448-451.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

PCR Amplification with Primers Based on IS2404 and GC-Rich Repeated Sequence Reveals Polymorphism in Mycobacterium ulcerans

Anthony Ablordey,1 Roman Kotlowski,1,2 Jean Swings,3 and Françoise Portaels1*

Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp,1 Laboratorium voor Microbiologie, Universiteit Gent, Gent, Belgium,3 Faculty of Chemistry, Gdansk University of Technology, Gdansk, Poland2

Received 8 June 2004/ Returned for modification 28 July 2004/ Accepted 15 September 2004

We describe a simple genotyping method for Mycobacterium ulcerans based on PCR amplification of genomic regions between IS2404 and a frequently repeated GC-rich sequence. Application of this method to a global collection produced 10 M. ulcerans genotypes corresponding to their geographic origin.

* Corresponding author. Mailing address: Mycobacteriology Unit, Institute of Tropical Medicine, B-2000 Antwerp, Belgium. Phone: 32-3-247-63-17. Fax: 32-3-247-63-33. E-mail: portaels{at}itg.be.

Journal of Clinical Microbiology, January 2005, p. 448-451, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.448-451.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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