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Journal of Clinical Microbiology, January 2005, p. 479-483, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.479-483.2005

Preliminary Evaluation of the API 20NE and RapID NF Plus Systems for Rapid Identification of Burkholderia pseudomallei and B. mallei

Mindy B. Glass* and Tanja Popovic

Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

Received 22 June 2004/ Returned for modification 1 September 2004/ Accepted 8 September 2004

We evaluated the API 20NE and the RapID NF Plus systems with 58 Burkholderia pseudomallei and 23 B. mallei strains for identification of these agents, but neither was reliable for confirmatory identification, with only 0 to 60% strains identified accurately. A greater diversity of strains in the system databases would be beneficial.

* Corresponding author. Mailing address: Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, CDC, MS G34, 1600 Clifton Rd., N.E., Atlanta, GA 30333. Phone: (404) 639-4055. Fax: (404) 639-3023. E-mail: mglass{at}cdc.gov.

Journal of Clinical Microbiology, January 2005, p. 479-483, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.479-483.2005




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