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Journal of Clinical Microbiology, January 2005, p. 488-490, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.488-490.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Analysis of Replication Kinetics of the Human Metapneumovirus in Different Cell Lines by Real-Time PCR

Céline Deffrasnes, Stéphanie Côté, and Guy Boivin*

Research Center in Infectious Diseases of the "Centre Hospitalier Universitaire de Québec" and Laval University, Quebec, Canada

Received 27 July 2004/ Returned for modification 13 September 2004/ Accepted 18 September 2004

Human metapneumovirus (hMPV) is associated with acute respiratory tract disease especially in young children. Using a quantitative real-time TaqMan PCR, we analyzed the replication kinetics of hMPV in different cell lines. Our results indicate that hMPV replicates slightly more efficiently in LLC-MK2 than in Vero cells and poorly in HEp-2 cells.

* Corresponding author. Mailing address: CHUQ-CHUL, Room RC-709, 2705 Blvd. Laurier, Sainte-Foy, Québec, Canada G1V 4G2. Phone: (418) 654-2705. Fax: (418) 654-2715. E-mail: Guy.Boivin{at}crchul.ulaval.ca.

Journal of Clinical Microbiology, January 2005, p. 488-490, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.488-490.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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