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Journal of Clinical Microbiology, January 2005, p. 494-496, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.494-496.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Discrepancies and Interpretation Problems in Susceptibility Testing of VIM-1-Producing Klebsiella pneumoniae Isolates

P. Giakkoupi,1 L. S. Tzouvelekis,2 G. L. Daikos,3 V. Miriagou,4 G. Petrikkos,3 N. J. Legakis,2 and A. C. Vatopoulos1*

Department of Microbiology, National School of Public Health,1 Department of Microbiology,2 1st Department of Propaedeutic Medicine, Medical School of Athens,3 Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece4

Received 21 July 2004/ Returned for modification 21 September 2004/ Accepted 27 September 2004

Susceptibilities to ß-lactam antibiotics of five VIM-1-producing Klebsiella pneumoniae isolates were determined by broth microdilution, Etest, disk diffusion, and the automated systems Vitek 2, Phoenix, and MicroScan. Significant discrepancies were observed in the determination of susceptibility to imipenem and meropenem. Interpretation problems by the automated systems were also noted.

* Corresponding author. Mailing address: Department of Microbiology, National School of Public Health, 196 Alexandras Ave., Athens 11521, Greece. Phone: 30 210 6422278. Fax: 30 210 6454002. E-mail: avatopou{at}nsph.gr.

Journal of Clinical Microbiology, January 2005, p. 494-496, Vol. 43, No. 1
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.1.494-496.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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