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Journal of Clinical Microbiology, October 2005, p. 5332-5337, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5332-5337.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of Universal Probes and Primer Sets for Assessing Total Bacterial Load in Clinical Samples: General Implications and Practical Use in Endodontic Antimicrobial Therapy

H. P. Horz,1 M. E. Vianna,1,2 B. P. F. A. Gomes,2 and G. Conrads1*

Division of Oral Microbiology and Immunology, Department of Operative and Preventive Dentistry & Periodontology, and Department of Medical Microbiology, RWTH Aachen University Hospital, Aachen, Germany,1 Endodontic Area, Department of Restorative Dentistry, Piracicaba Dental School, State University of Campinas, Piracicaba, SP, Brazil2

Received 16 February 2005/ Returned for modification 26 April 2005/ Accepted 20 July 2005

By reexamining 10 previously published "universal" PCR assays using the ARB phylogenetic software package and database with 41,000 16S rRNA gene sequences, we found that they differed considerably in their coverage of the domain Bacteria. We evaluated the broadest-range real-time quantitative PCR protocol for its efficacy in measuring the antimicrobial effects of endodontic treatments.

* Corresponding author. Mailing address: Division of Oral Microbiology and Immunology, University Hospital (RWTH), Pauwelsstrasse 30, D-52057 Aachen, Germany. Phone: 49-241-9632141. Fax: 49-241-8082483. E-mail: gconrads{at}ukaachen.de.

Journal of Clinical Microbiology, October 2005, p. 5332-5337, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5332-5337.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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