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Journal of Clinical Microbiology, October 2005, p. 5355-5358, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5355-5358.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Tularemia in Denmark: Identification of a Francisella tularensis subsp. holarctica Strain by Real-Time PCR and High-Resolution Typing by Multiple-Locus Variable-Number Tandem Repeat Analysis

Mona Byström,1 Sidsel Böcher,2,{dagger} Anna Magnusson,1,3,4 Jørgen Prag,2 and Anders Johansson1,3*

Department of NBC-Analysis, Swedish Defence Research Agency, Umeå, Sweden,1 Department of Clinical Microbiology, Viborg Hospital, Viborg, Denmark,2 Department of Clinical Microbiology, Infectious Diseases, Umeå University, Umeå, Sweden,3 Department of Infectious Diseases, Sundsvall Hospital, Sundsvall, Sweden4

Received 14 March 2005/ Returned for modification 30 April 2005/ Accepted 10 July 2005

We report ulceroglandular tularemia affecting an 8-year-old boy and the first recovery of Francisella tularensis in Denmark. A novel real-time PCR assay was used to identify the strain as F. tularensis subsp. holarctica (type B). Multiple-locus variable-number tandem repeat analysis demonstrated a close genetic relationship to strains from Norway.

* Corresponding author. Mailing address: NBC-Analysis, Swedish Defence Research Agency, SE-901 82 Umeå, Sweden. Phone: 46 90 10 66 16. Fax: 46 90 13 30 06. E-mail: anders.johansson{at}infdis.umu.se.

{dagger} Present address: National Center for Antimicrobials and Infection Control, Statens Serum Institut, Copenhagen, Denmark.

Journal of Clinical Microbiology, October 2005, p. 5355-5358, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5355-5358.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Marinov, K. T., Georgieva, E. D., Ivanov, I. N., Kantardjiev, T. V. (2009). Characterization and genotyping of strains of Francisella tularensis isolated in Bulgaria. J Med Microbiol 58: 82-85 [Abstract] [Full Text]  


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