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Journal of Clinical Microbiology, October 2005, p. 5366-5368, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5366-5368.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Development of a Nested Qualitative Real-Time PCR Assay To Detect Aspergillus Species DNA in Clinical Specimens

Catriona Halliday,1,2,3* Qi Xuan Wu,1,3 Gregory James,3 and Tania Sorrell1,2

Centre for Infectious Diseases and Microbiology & NHMRC Clinical Centre of Research Excellence in Infectious Diseases, Hematology, and Health Ethics, University of Sydney Western Clinical School,1 Westmead Hospital,2 Centre for Infectious Diseases and Microbiology Laboratory Services, Westmead Hospital, Westmead, New South Wales 2145, Australia3

Received 17 May 2005/ Returned for modification 2 June 2005/ Accepted 6 July 2005

We adapted a nested Aspergillus PCR to a nested qualitative real-time format on a LightCycler system. An evaluation using 134 clinical specimens showed that the real-time PCR assay significantly reduced the time for results to be made available without compromising sensitivity and was less labor-intensive. Its use will aid in the rapid diagnosis of invasive aspergillosis.

* Corresponding author. Mailing address: Centre for Infectious Diseases and Microbiology, Westmead Hospital, Westmead, New South Wales 2145, Australia. Phone: 61-2-9845 6255. Fax: 61-2-9893 8659. E-mail: catrionah{at}icpmr.wsahs.nsw.gov.au.

Journal of Clinical Microbiology, October 2005, p. 5366-5368, Vol. 43, No. 10
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.10.5366-5368.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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