This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Skoczynska, A.
Right arrow Articles by Hryniewicz, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Skoczynska, A.
Right arrow Articles by Hryniewicz, W.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2005, p. 5665-5669, Vol. 43, No. 11
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.11.5665-5669.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Characteristics of Haemophilus influenzae Type b Responsible for Meningitis in Poland from 1997 to 2004

Anna Skoczynska,1* Marcin Kadlubowski,1 Joanna Empel,2 and Waleria Hryniewicz1

National Reference Centre for Bacterial Meningitis, Department of Epidemiology and Clinical Microbiology,1 Department of Molecular Microbiology, National Institute of Public Health, Warsaw, Poland2

Received 13 July 2005/ Returned for modification 1 August 2005/ Accepted 6 September 2005

Two hundred forty-five H. influenzae isolates responsible for meningitis in Poland from 1997 to 2004 were studied. Among these, 233 (95.1%) belonged to serotype b (Hib), 2 belonged to serotype f, and 10 were noncapsulated. The relatedness of all isolates was evaluated by pulsed-field gel electrophoresis (PFGE), and selected representatives were evaluated by multilocus sequence typing. Resistance to ampicillin was identified in 34 (14.6%) of the Hib isolates and was associated with the production of ß-lactamase only. Except for four isolates nonsusceptible to chloramphenicol, all isolates were susceptible to cefotaxime, ciprofloxacin, and rifampin. The PFGE analysis divided the Hib isolates into five PFGE types; however, all of them were possibly related. The most common PFGE type, with 25 subtypes, was characteristic for 97.4% of the isolates. The most prevalent PFGE subtype found in our study was also the most common among the Hib isolates responsible for invasive disease in Italy and the Czech Republic and was found among isolates causing lower respiratory tract infections in Poland. The most prevalent sequence types (STs) in the studied group were ST6 and ST92. Four new STs were found: ST188, ST189, ST190, and ST268. Results of this study support the evidence that the genetic structure of encapsulated H. influenzae is clonal. The continuing high number of meningitis cases due to Hib in Poland underlines the need for mass vaccination against Hib in Poland.

* Corresponding author. Mailing address: National Reference Centre for Bacterial Meningitis, Dept. of Epidemiology and Clinical Microbiology, National Institute of Public Health, Chelmska 30/34, 00-725 Warsaw, Poland. Phone: 48 22 8514670. Fax: 48 22 8412949. E-mail: skoczek{at}cls.edu.pl.

Journal of Clinical Microbiology, November 2005, p. 5665-5669, Vol. 43, No. 11
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.11.5665-5669.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Petris, C. K., Golomb, M., Phillips, T. E. (2007). Bacterial Transcytosis across Conjunctival M Cells. IOVS 48: 2172-2177 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»