This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nissley, D. V.
Right arrow Articles by Strathern, J. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nissley, D. V.
Right arrow Articles by Strathern, J. N.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2005, p. 5696-5704, Vol. 43, No. 11
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.11.5696-5704.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Sensitive Phenotypic Detection of Minor Drug-Resistant Human Immunodeficiency Virus Type 1 Reverse Transcriptase Variants

Dwight V. Nissley,1,3* Elias K. Halvas,2 Nicole L. Hoppman,3 David J. Garfinkel,3 John W. Mellors,2 and Jeffrey N. Strathern3

Basic Research Program, SAIC-Frederick, Inc., NCI Frederick, Frederick, Maryland,1 Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania,2 Gene Regulation and Chromosome Biology Laboratory, NCI Frederick, Frederick, Maryland3

Received 4 November 2004/ Returned for modification 2 May 2005/ Accepted 4 August 2005

Detection of drug-resistant variants is important for the clinical management of human immunodeficiency virus type 1 (HIV-1) infection and for studies on the evolution of drug resistance. Here we show that hybrid elements composed of the Saccharomyces cerevisiae retrotransposon Ty1 and the reverse transcriptase (RT) of HIV-1 are useful tools for detecting, monitoring, and isolating drug-resistant reverse transcriptases. This sensitive phenotypic assay is able to detect nonnucleoside reverse transcriptase inhibitor-resistant RT domains derived from mixtures of infectious molecular clones of HIV-1 in plasma and from clinical samples when the variants comprise as little as 0.3 to 1% of the virus population. Our assay can characterize the activities and drug susceptibilities of both known and novel reverse transcriptase variants and should prove useful in studies of the evolution and clinical significance of minor drug-resistant viral variants.

* Corresponding author. Mailing address: BRP, SAIC-Frederick, NCI Frederick, P.O. Box B, Frederick, MD 21702. Phone: (301) 846-1181. Fax: (301) 846-6911. E-mail: nissley{at}ncifcrf.gov.

Journal of Clinical Microbiology, November 2005, p. 5696-5704, Vol. 43, No. 11
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.11.5696-5704.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Svarovskaia, E. S., Moser, M. J., Bae, A. S., Prudent, J. R., Miller, M. D., Borroto-Esoda, K. (2006). MultiCode-RTx Real-Time PCR System for Detection of Subpopulations of K65R Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant Viruses in Clinical Samples. J. Clin. Microbiol. 44: 4237-4241 [Abstract] [Full Text]  
  • Halvas, E. K., Aldrovandi, G. M., Balfe, P., Beck, I. A., Boltz, V. F., Coffin, J. M., Frenkel, L. M., Hazelwood, J. D., Johnson, V. A., Kearney, M., Kovacs, A., Kuritzkes, D. R., Metzner, K. J., Nissley, D. V., Nowicki, M., Palmer, S., Ziermann, R., Zhao, R. Y., Jennings, C. L., Bremer, J., Brambilla, D., Mellors, J. W. (2006). Blinded, multicenter comparison of methods to detect a drug-resistant mutant of human immunodeficiency virus type 1 at low frequency.. J. Clin. Microbiol. 44: 2612-2614 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»