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Journal of Clinical Microbiology, November 2005, p. 5715-5720, Vol. 43, No. 11
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.11.5715-5720.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Distribution of tccP in Clinical Enterohemorrhagic and Enteropathogenic Escherichia coli Isolates{ddagger}

Junkal Garmendia,1,{dagger} Zhihong Ren,2,{dagger} Sharon Tennant,3,{dagger} Monica Aparecida Midolli Viera,4,{dagger} Yuwen Chong,5,{dagger} Andrew Whale,1,{dagger} Kristy Azzopardi,7 Sivan Dahan,1 Marcelo Palma Sircili,6 Marcia Regina Franzolin,6 Luiz R. Trabulsi,6 Alan Phillips,5 Tânia A. T. Gomes,4 Jianguo Xu,2 Roy Robins-Browne,3 and Gad Frankel1*

Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, London, United Kingdom,1 State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Diseases Control and Prevention, China CDC, Beijing, China,2 Department of Microbiology and Immunology; University of Melbourne,3 Murdoch Childrens Research Institute, Royal Children's Hospital, Victoria, Australia,7 Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo, Sao Paulo, Brazil,4 Centre for Paediatric Gastroenterology, Royal Free and University College Medical School, London, United Kingdom,5 Laboratorio Especial de Microbiologia, Instituto Butanta, Sao Paulo, Brazil6

Received 14 April 2005/ Returned for modification 7 July 2005/ Accepted 17 August 2005

Enterohemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC) are diarrheagenic pathogens that colonize the gut through the formation of attaching and effacing lesions, which depend on the translocation of effector proteins via a locus of enterocyte effacement-encoded type III secretion system. Recently, two effector proteins, EspJ and TccP, which are encoded by adjacent genes on prophage CP-933U in EHEC O157:H7, have been identified. TccP consists of a unique N-terminus region and several proline-rich domains. In this project we determined the distribution of tccP in O157:H7, in non-O157 EHEC, and in typical and atypical EPEC isolates. All the EHEC O157:H7 strains tested were tccP+. Unexpectedly, tccP was also found in non-O157 EHEC, and in typical and atypical EPEC isolates, particularly in strains belonging to serogroups O26 (EHEC), O119 (typical EPEC), and O55 (atypical EPEC). We recorded some variation in the length of tccP, which reflects diversity in the number of the proline-rich repeats. These results show the existence of a class of "attaching and effacing" pathogens which express a combination of EPEC and EHEC virulence determinants.

* Corresponding author. Mailing address: Centre for Molecular Microbiology and Infection, Flowers Building, Division of Molecular and Cellular Biology, Imperial College London, London SW7 2AZ, United Kingdom. Phone: 44 (0) 207594525. Fax: 44 (0) 2075943069. E-mail: g.frankel{at}imperial.ac.uk.

{ddagger} This study is in memory of Luiz R. Trabulsi, who passed away in June 2005.

{dagger} J.G., Z.R., S.T., M.A.M.V., Y.C., and A.W. contributed equally to this study.

Journal of Clinical Microbiology, November 2005, p. 5715-5720, Vol. 43, No. 11
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.11.5715-5720.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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