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Journal of Clinical Microbiology, November 2005, p. 5771-5774, Vol. 43, No. 11
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.11.5771-5774.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of a Real-Time PCR TaqMan Assay for Rapid Identification and Differentiation of Burkholderia pseudomallei and Burkholderia mallei

Jana M. U'Ren,1 Matthew N. Van Ert,1 James M. Schupp,1 W. Ryan Easterday,1 Tatum S. Simonson,1 Richard T. Okinaka,2 Talima Pearson,1 and Paul Keim1*

Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona 86011-5640,1 Los Alamos National Laboratory, Los Alamos, New Mexico 875452

Received 8 June 2005/ Returned for modification 19 July 2005/ Accepted 24 August 2005

A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.

* Corresponding author. Mailing address: Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ 86011-5640. Phone: (928) 523-1078. Fax: (928) 523-0639. E-mail: Paul.Keim{at}nau.edu.

Journal of Clinical Microbiology, November 2005, p. 5771-5774, Vol. 43, No. 11
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.11.5771-5774.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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