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Journal of Clinical Microbiology, December 2005, p. 5925-5935, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.5925-5935.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Polyphasic Characterization Reveals that the Human Pathogen Mycobacterium peregrinum Type II Belongs to the Bovine Pathogen Species Mycobacterium senegalense{dagger}

Richard J. Wallace Jr.,1* Barbara A. Brown-Elliott,1 June Brown,2 Arnold G. Steigerwalt,2 Leslie Hall,3 Gail Woods,4 Joann Cloud,4 Linda Mann,1 Rebecca Wilson,1 Christopher Crist,1 Kenneth C. Jost Jr.,5 Dorothy E. Byrer,6,{ddagger} Jane Tang,6 Jason Cooper,6 Elena Stamenova,6 Brian Campbell,1,§ Joyce Wolfe,7 and Christine Turenne7

Department of Microbiology and Mycobacteria/Nocardia Research Laboratory, The University of Texas Health Center, Tyler, Texas,1 Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia,2 Mayo Clinic, Rochester, Minnesota,3 Department of Pathology, University of Utah, Salt Lake City, Utah,4 Texas Department of Health, Austin, Texas,5 American Type Culture Collection, Manassas, Virginia,6 National Reference Center for Mycobacteriology, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada7

Received 18 March 2005/ Returned for modification 30 May 2005/ Accepted 15 August 2005

Mycobacterium peregrinum consists of two taxa: types I and II. We evaluated 43 clinical type II strains from throughout the United States. They were responsible for soft-tissue and bone infections, catheter-related infections, and possible pneumonitis. By carbohydrate utilization, they were indistinguishable from type I strains, being D-mannitol and trehalose positive. However, they had a distinct susceptibility pattern that included intermediate ciprofloxacin MICs but low clarithromycin and doxycycline MICs of ≤1 µg/ml. These features were also shared by reference isolates of Mycobacterium senegalense from African bovine cases of "farcy." By 16S rRNA gene sequencing, the type II isolates shared 100% sequence identity with M. senegalense. Partial sequencing of the type II hsp65 gene (441 bp) revealed four sequevars showing ≥98.4% identity with each other and ≥98.6% identity with the sequence of five bovine strains of M. senegalense. There was ≤97.1% identity with M. peregrinum type I isolates and other Mycobacterium fortuitum group species. Sequencing of additional gene targets including the 16S-23S rDNA internal transcribed spacer region and the rpoB gene (partial sequence) revealed a similar phylogenetic grouping. DNA-DNA hybridization showed 76 to 99% relatedness between the bovine and human strains. These studies demonstrate that type II isolates are not isolates of M. peregrinum but represent human strains of M. senegalense. This study is the first to demonstrate this species as a human pathogen. Representative human M. senegalense strains include ATCC 35755 and newly submitted strains ATCC BAA-849, ATCC BAA-850, and ATCC BAA-851.

* Corresponding author. Mailing address: The University of Texas Health Center, Department of Microbiology, 11937 US Highway 271, Tyler, TX 75708. Phone: (903) 877-7680. Fax: (903) 877-7652. E-mail: richard.wallace{at}uthct.edu.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

{ddagger} Present address: Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, GA 30333.

§ Present address: Texas A&M University, College Station, Texas.

Journal of Clinical Microbiology, December 2005, p. 5925-5935, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.5925-5935.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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