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Journal of Clinical Microbiology, December 2005, p. 6136-6138, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6136-6138.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Broth Microdilution Susceptibility Testing of Campylobacter jejuni and the Determination of Quality Control Ranges for Fourteen Antimicrobial Agents

Patrick F. McDermott,1* Sonya M. Bodeis-Jones,1 Thomas R. Fritsche,2 Ronald N. Jones,2 Robert D. Walker,1 and the Campylobacter Susceptibility Testing Group

Food and Drug Administration, Office of Research, Center for Veterinary Medicine, Laurel, Maryland 20708,1 JMI Laboratories, North Liberty, Iowa 523172

Received 20 July 2005/ Returned for modification 10 September 2005/ Accepted 1 October 2005

Quality control ranges were developed for broth microdilution testing of Campylobacter jejuni ATCC 33560 against 14 antimicrobials. Cation-adjusted Mueller-Hinton broth containing 2.5% laked horse blood was the preferred medium, with incubation in a microaerobic atmosphere of 10% CO2, 5% O2, and 85% N2 at 36°C for 48 h or 42°C for 24 h.

* Corresponding author. Mailing address: U.S. Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Road, Laurel, MD 20708. Phone: (301) 210-4213. Fax: (301) 210-4298. E-mail: Patrick.McDermott{at}fda.gov.

Journal of Clinical Microbiology, December 2005, p. 6136-6138, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6136-6138.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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