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Journal of Clinical Microbiology, December 2005, p. 6144-6146, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6144-6146.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of the LightCycler Staphylococcus MGRADE Kits on Positive Blood Cultures That Contained Gram-Positive Cocci in Clusters

Nabin K. Shrestha,1* Marion J. Tuohy,2 Ravindran A. Padmanabhan,1 Gerri S. Hall,2 and Gary W. Procop2

Department of Infectious Diseases,1 Section of Clinical Microbiology, The Cleveland Clinic Foundation, Cleveland, Ohio2

Received 10 August 2005/ Returned for modification 8 September 2005/ Accepted 5 October 2005

We evaluated the Roche LightCycler Staphylococcus MGRADE kits to differentiate between Staphylococcus aureus and coagulase-negative staphylococci in blood cultures growing clusters of gram-positive cocci. Testing 100 bottles (36 containing S. aureus), the assay was 100% sensitive and 98.44% specific for S. aureus and 100% sensitive and specific for coagulase-negative staphylococci.

* Corresponding author. Mailing address: Department of Infectious Diseases, The Cleveland Clinic Foundation, 9500 Euclid Avenue, S-32, Cleveland, OH 44195. Phone: (216) 444-1687. Fax: (216) 445-9446. E-mail: shrestn{at}ccf.org.

Journal of Clinical Microbiology, December 2005, p. 6144-6146, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6144-6146.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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