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Journal of Clinical Microbiology, December 2005, p. 6147-6149, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6147-6149.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Development of a Triplex Real-Time PCR Assay for Detection of Panton-Valentine Leukocidin Toxin Genes in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

Ryan R. McDonald,1* Nick A. Antonishyn,1 Toni Hansen,1 Laelie A. Snook,1,{dagger} Evelyn Nagle,1 Michael R. Mulvey,2 Paul N. Levett,1 and Greg B. Horsman1

Provincial Laboratory, Saskatchewan Health, Regina, Saskatchewan, Canada,1 National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada2

Received 21 September 2005/ Accepted 4 October 2005

Community-associated methicillin-resistant Staphylococcus aureus harboring Panton-Valentine leukocidin (PVL) genes is an emerging pathogen. A novel real-time PCR assay for identification of MRSA isolates containing PVL was developed. The PVL assay was used in a triplex format allowing simultaneous amplification of mecA, nuc, and PVL genes in 614 clinical isolates. This assay facilitates the rapid identification of PVL-positive isolates of MRSA.

* Corresponding author. Mailing address: Provincial Laboratory, Saskatchewan Health, 3211 Albert St., Regina, Saskatchewan S4S 5W6, Canada. Phone: (306) 787-1961. Fax: (306) 787-9122. E-mail: rmcdonal{at}health.gov.sk.ca.

{dagger} Present address: Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada.

Journal of Clinical Microbiology, December 2005, p. 6147-6149, Vol. 43, No. 12
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.12.6147-6149.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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