This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van der Heide, R.
Right arrow Articles by van der Poel, W. H. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van der Heide, R.
Right arrow Articles by van der Poel, W. H. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2005, p. 669-675, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.669-675.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Molecular Characterizations of Human and Animal Group A Rotaviruses in The Netherlands

R. van der Heide,1 M. P. G. Koopmans,2 N. Shekary,1 D. J. Houwers,3 Y. T. H. P. van Duynhoven,4 and W. H. M. van der Poel1*

Microbiological Laboratory for Health Protection,1 Diagnostic Laboratory for Infectious Diseases and Perinatal Screening,2 Department of Infectious Diseases Epidemiology, National Institute for Public Health and the Environment, Bilthoven,4 Veterinary Microbiological Diagnostic Centre, Utrecht University, Utrecht, The Netherlands3

Received 3 February 2004/ Returned for modification 3 April 2004/ Accepted 4 July 2004

To gain more insight into interspecies transmission of rotavirus group A, human and animal fecal samples were collected between 1997 and 2001 in The Netherlands. A total of 110 human stool samples were successfully P and G genotyped by reverse transcriptase PCR. All strains belonged to the main human rotavirus genotypes G1 to G4, G9, [P4], [P6], [P8], and [P9]. [P8]G1 was predominant, and 5.5% belonged to the G9 genotype. Eleven percent of all P[8] genotypes could be genotyped only by a recently published modified primer. Rotavirus-positive fecal samples from 28 calf herds were genotyped by DNA sequencing. Genotypes G6 and G10 predominated; G6 and G10 were detected in 22 (78.6%) and 16 (57.1%) of the rotavirus-positive calf herds, respectively. In 12 (42.9%) calf herds, we found mixed infections. Genotype G8 was not found. Genotype G6 bovine rotaviruses were divided into three clusters: UK-like, VMRI-29-like, and Hun4-like. DNA sequencing of a part of the VP7 gene was shown to be useful as a quick determination of uncommon or novel strains of which the genotyping cannot be done by genotyping PCR. Of equine strains, both VP4 and VP7 genes could be used for genotyping: two [P12]G3 and four [P12]G14 equine rotaviruses were determined. We did not find indications for rotavirus interspecies transmissions, although the recently published human G6-Hun4 is genetically related to our G6 bovine isolates. All bovine, porcine, and equine rotaviruses were within genotypes previously reported for these animal species.

* Corresponding author. Present address: Animal Sciences Group, Wageningen University Research, Infectious Diseases Division, Department Infection Biology, P.O. Box 65, NL-8200 AB Lelystad, Edelhertweg 15, NL-8219 PH Lelystad, The Netherlands. Phone: 31 320 238383. Fax: 31 320 238961. E-mail: wim.vanderpoel{at}wur.nl.

Journal of Clinical Microbiology, February 2005, p. 669-675, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.669-675.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Steyer, A., Poljsak-Prijatelj, M., Barlic-Maganja, D., Marin, J. (2008). Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment. J. Gen. Virol. 89: 1690-1698 [Abstract] [Full Text]  
  • van Zyl, W. B., Page, N. A., Grabow, W. O. K., Steele, A. D., Taylor, M. B. (2006). Molecular epidemiology of group a rotaviruses in water sources and selected raw vegetables in southern Africa.. Appl. Environ. Microbiol. 72: 4554-4560 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»