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Journal of Clinical Microbiology, February 2005, p. 755-760, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.755-760.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Detection and Characterization of Diarrheagenic Escherichia coli from Young Children in Hanoi, Vietnam

Trung Vu Nguyen,1,2 Phung Le Van,1 Chinh Le Huy,1 Khanh Nguyen Gia,3 and Andrej Weintraub2*

Department of Medical Microbiology,1 Department of Pediatrics, Hanoi Medical University, Hanoi, Vietnam,3 Department of Laboratory Medicine, Division of Clinical Bacteriology, Karolinska Institutet, Karolinska University Hospital, Huddinge, Stockholm, Sweden2

Received 30 July 2004/ Returned for modification 1 October 2004/ Accepted 6 October 2004

Diarrhea continues to be one of the most common causes of morbidity and mortality among infants and children in developing countries. Escherichia coli is an emerging agent among pathogens that cause diarrhea. The development of a highly applicable technique for the detection of different categories of diarrheagenic E. coli is important. We have used multiplex PCR by combining eight primer pairs specific for enteroaggregative E. coli (EAEC), enteroinvasive E. coli (EIEC), enterohemorrhagic E. coli, enteropathogenic E. coli (EPEC), and enterotoxigenic E. coli (ETEC). This facilitates the identification of five different categories of diarrheagenic E. coli from stool samples in a single reaction simultaneously. The prevalences of diarrheagenic E. coli were 22.5 and 12% in the diarrhea group and the control group, respectively. Among 587 fecal samples from Vietnamese children under 5 years of age with diarrhea, this technique identified 132 diarrheagenic E. coli strains. This included 68 samples (11.6%) with EAEC, 12 samples (2.0%) with EIEC, 39 samples (6.6%) with EPEC, and 13 samples (2.2%) with ETEC. Among the 249 age-matched controls, 30 samples were positive for diarrheagenic E. coli. The distribution was 18 samples (7.2%) with EAEC, 11 samples (4.4%) with EPEC, and 1 sample (0.4%) with ETEC.

* Corresponding author. Mailing address: Department of Laboratory Medicine, Division of Clinical Bacteriology, Karolinska Institutet, F-82 Karolinska University Hospital, Huddinge, S-141 86 Stockholm, Sweden. Phone: 46 8 585 87831. Fax: 46 8 711 3918. E-mail: andrej.weintraub{at}labmed.ki.se.

Journal of Clinical Microbiology, February 2005, p. 755-760, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.755-760.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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