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Journal of Clinical Microbiology, February 2005, p. 896-897, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.896-897.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of a New Selective Enrichment Broth for Detection of Group B Streptococci in Pregnant Women

Judith S. Heelan,1,2* Judith Struminsky,3 Patricia Lauro,3 and C. James Sung2

Departments of Pathology and Laboratory Medicine, Memorial Hospital of Rhode Island, Pawtucket,1 Brown Medical School,2 Women and Infants Hospital of Rhode Island, Providence, Rhode Island3

Received 26 July 2004/ Returned for modification 15 October 2004/ Accepted 18 October 2004

Studies at two Brown Medical School-affiliated hospitals were undertaken to evaluate a new selective broth medium (GBS broth) and to compare it to the LIM broth currently used to culture for group B streptococci. Beta-hemolytic group B streptococci produce a carotenoid pigment that turns GBS broth an orange color. From a total of 580 pregnant women, duplicate vaginal-rectal swabs were collected at 35 to 37 weeks of gestation and cultured for group B streptococci, using either LIM broth (a selective broth containing antibiotics) or GBS broth for enrichment. Specimens were either transported to the laboratory or immediately placed in the respective enrichment broths and delivered to the laboratory. GBS broth medium had sensitivity, specificity, and positive and negative predictive values of 87.8, 100, 100, and 95.1% when planted in the laboratory and 90.3, 100, 100 and 97.6%, respectively, when inoculated at bedside. Use of GBS broth would satisfy Centers for Disease Control and Prevention requirements and would provide faster, more-sensitive, and cost-effective detection of group B streptococci in pregnant women.

* Corresponding author. Mailing address: Memorial Hospital of Rhode Island, 111 Brewster St., Pawtucket, RI 02860. Phone: (401) 729-2839. Fax: (401) 729-2990. E-mail: jheelan{at}mhri.org.

Journal of Clinical Microbiology, February 2005, p. 896-897, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.896-897.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • de la Rosa-Fraile, M., Camacho-Munoz, E. (2005). Liquid Granada Medium for Detection of Group B Streptococci. J. Clin. Microbiol. 43: 4303-4303 [Full Text]  


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