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Journal of Clinical Microbiology, February 2005, p. 903-905, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.903-905.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Reliability of the E-Test Method for Detection of Colistin Resistance in Clinical Isolates of Acinetobacter baumannii

L. A. Arroyo,1* A. García-Curiel,1 M. E. Pachón-Ibañez,2 A. C. Llanos,1 M. Ruiz,1 J. Pachón,2 and J. Aznar1

Clinical Microbiology,1 Infectious Diseases Services, University Hospitals Vírgen del Rocío, Seville, Spain2

Received 22 April 2004/ Returned for modification 3 June 2004/ Accepted 28 September 2004

We compared the E-test to the broth microdilution method for testing the susceptibility of 115 clinical isolates of Acinetobacter baumannii to colistin. Twenty-two (19.1%) strains were resistant to colistin and 93 (80.8%) strains were susceptible according to the reference broth microdilution method. A categorical agreement of 98.2% was found, with only two (1.7%) very major errors. Agreement within 1 twofold dilution between the E-test and the broth microdilution was 16.5%. Complete agreement was found for the strains for which MICs fell within the range of 0.25 to 1 µg of colistin/ml. However, there was poor concordance, particularly in extreme dilutions with higher MICs by the E-test method.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospitales Universitarios Vírgen del Rocío, Avda. Manuel Siurot, s/n, 41013 Seville, Spain. Phone: 34 955013204. Fax: 34 955013211. E-mail:larroyo{at}us.es.

Journal of Clinical Microbiology, February 2005, p. 903-905, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.903-905.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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