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Journal of Clinical Microbiology, February 2005, p. 909-912, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.909-912.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of Amplified Fragment Length Polymorphism for Differentiation of Avian Mycoplasma Species

Yang Hong,1 Maricarmen García,1* Sharon Levisohn,2 Inna Lysnyansky,2 Victoria Leiting,1 P. H. M. Savelkoul,3 and S. H. Kleven1

Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia,1 Division of Avian & Aquatic Diseases, Kimron Veterinary Institute, Bet Dagan, Israel,2 Department of Medical Microbiology and Infection Control, University Hospital Vrije Universiteit, Amsterdam, The Netherlands3

Received 11 June 2004/ Accepted 6 September 2004

Amplified fragment length polymorphism (AFLP) was used for typing avian mycoplasma species. Forty-four avian mycoplasma strains were successfully typed into eight distinct groups, with each representing a different species. Homology of AFLP patterns of 35% or less was used as a cutoff value to differentiate avian mycoplasma strains into different species.

* Corresponding author. Mailing address: Department of Avian Medicine, College of Veterinary Medicine, University of Georgia, 953 College Station Rd., Athens, GA 30602. Phone: (706) 542-5656. Fax: (706) 542-5630. E-mail: mcgarcia{at}uga.edu.

Journal of Clinical Microbiology, February 2005, p. 909-912, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.909-912.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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