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Journal of Clinical Microbiology, February 2005, p. 948-958, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.948-950.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Duplex PCR To Differentiate between Mycoplasma synoviae and Mycoplasma gallisepticum on the Basis of Conserved Species-Specific Sequences of Their Hemagglutinin Genes

B. Ben Abdelmoumen Mardassi,* R. Ben Mohamed, I. Gueriri, S. Boughattas, and B. Mlik

Laboratoire des Mycoplasmes, Institut Pasteur de Tunis, Tunis, Tunisia

Received 5 February 2004/ Returned for modification 2 April 2004/ Accepted 8 October 2004

We developed a duplex PCR assay targeting the hemagglutinin multigene families, vlhA and pMGA, of Mycoplasma synoviae and Mycoplasma gallisepticum, respectively. The assay proved to be specific and sensitive enough to justify its use for the simultaneous detection of the two major avian mycoplasma species from field isolates.

* Corresponding author. Mailing address: Institut Pasteur de Tunis, Laboratoire des Mycoplasmes, 13 Place Pasteur-B.P. 74, 1002 Tunis-Belvédère, Tunis, Tunisia. Phone: (216) 71-790-921, ext. 342. Fax: (216) 71-791-833. E-mail: boutheina.mardassi{at}pasteur.rns.tn.

Journal of Clinical Microbiology, February 2005, p. 948-958, Vol. 43, No. 2
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.2.948-950.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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