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Journal of Clinical Microbiology, March 2005, p. 1059-1063, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1059-1063.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparison of Specimen Processing and Nucleic Acid Extraction by the Swab Extraction Tube System versus the MagNA Pure LC System for Laboratory Diagnosis of Herpes Simplex Virus Infections by LightCycler PCR

N. C. Issa,1 M. J. Espy,1 J. R. Uhl,1 W. S. Harmsen,2 J. N. Mandrekar,2 R. E. Gullerud,2 M. D. Davis,3 and T. F. Smith1*

Division of Clinical Microbiology,1 Department of Health Sciences Research,2 Department of Dermatology, Mayo Clinic College of Medicine, Rochester, Minnesota3

Received 19 July 2004/ Returned for modification 14 September 2004/ Accepted 9 November 2004

A total of 563 specimens (234 dermal and 329 genital swabs) from patients suspected of having herpes simplex virus (HSV) infections were processed using two different extraction methods (the MagNA Pure LC system and the swab extraction tube system [SETS]); HSV DNA was amplified by LightCycler PCR. HSV DNA was detected in 157 of 563 specimens (27.9%) processed by the MagNA Pure LC system and in 179 of 563 specimens (31.8%) processed by SETS (P < 0.0001). There was no specimen processed by the MagNA Pure LC extraction method that was positive only for HSV DNA. Of 157 specimens positive by both methods, HSV DNA copy levels were higher (using cycle crossover points [cycle threshold {CT}]) with SETS (mean CT, 25.9 cycles) than with the MagNA Pure LC system (mean CT, 32.0 cycles) (P < 0.0001). The time to process 32 samples was longer with the MagNA Pure LC extraction system (90 min) than with SETS (35 min). HSV DNA extraction using SETS is faster, less expensive, and more sensitive than the MagNA Pure LC system and could replace the latter for the laboratory diagnosis of HSV infections using LightCycler PCR.

* Corresponding author. Mailing address: Division of Clinical Microbiology, Mayo Clinic, 200 First St., SW, Rochester, MN 55905. Phone: (507) 284-8146. Fax: (507) 284-4272. E-mail: tfsmith{at}mayo.edu.

Journal of Clinical Microbiology, March 2005, p. 1059-1063, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1059-1063.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dundas, N., Leos, N. K., Mitui, M., Revell, P., Rogers, B. B. (2008). Comparison of Automated Nucleic Acid Extraction Methods with Manual Extraction. J. Mol. Diagn. 10: 311-316 [Abstract] [Full Text]  
  • Espy, M. J., Uhl, J. R., Sloan, L. M., Buckwalter, S. P., Jones, M. F., Vetter, E. A., Yao, J. D. C., Wengenack, N. L., Rosenblatt, J. E., Cockerill, F. R. III, Smith, T. F. (2006). Real-Time PCR in Clinical Microbiology: Applications for Routine Laboratory Testing. Clin. Microbiol. Rev. 19: 165-256 [Abstract] [Full Text]  


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