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Journal of Clinical Microbiology, March 2005, p. 1064-1068, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1064-1068.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Multiplex PCR Assay for Rapid Identification of Oculopathogenic Adenoviruses by Amplification of the Fiber and Hexon Genes

Urmila Banik ,1,{dagger} Arun Kumar Adhikary,1* Eiko Suzuki,2 Toshiki Inada,1 and Nobuhiko Okabe1

Infectious Disease Surveillance Center, National Institute of Infectious Diseases,1 Department of Developmental Medical Sciences, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan2

Received 8 August 2004/ Returned for modification 21 August 2004/ Accepted 13 September 2004

Eye infections caused by adenovirus (Ad) often result in nosocomial infections and community epidemics with significant rates of morbidity. No antiviral agent effective against Ad is yet available for clinical use. Therefore, early diagnosis is still the mainstay for patient management and the prevention of epidemics. A multiplex PCR assay based on amplification of a combination of the fiber and hexon genes which can identify the six important oculopathogenic serotypes of Ads (Ad serotype 3[Ad3], Ad4, Ad7, Ad8, Ad19, and Ad37) in a single-tube amplification reaction was developed. Ad serotypes could be distinguished by the different amplicon sizes. The assay correctly identified prototype strains as well as isolates in clinical specimens. In comparison with a previously described PCR-restriction fragment polymorphism method, our assay gave unequivocal results for clinical specimens. Our multiplex PCR has the potential to serve as a rapid and cost-effective tool for the typing of important ocular Ads.

* Corresponding author. Mailing address: Infectious Disease Surveillance Center, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. Phone: 81-3-5285-1111, ext. 2533. Fax: 81-3-5285-1129. E-mail: arun_adhikary{at}msn.com.

{dagger} Present address: Department of Medicine, Chittagong Medical College Hospital, Chittagong, Bangladesh.

Journal of Clinical Microbiology, March 2005, p. 1064-1068, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1064-1068.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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