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Journal of Clinical Microbiology, March 2005, p. 1234-1238, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1234-1238.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Identification of Streptococcus canis Isolated from Milk of Dairy Cows with Subclinical Mastitis

Abdulwahed Ahmed Hassan,* Ömer Akineden, and Ewald Usleber

Institut für Tierärztliche Nahrungsmittelkunde, Justus-Liebig-Universität Giessen, Giessen, Germany

Received 18 March 2004/ Returned for modification 22 August 2004/ Accepted 20 October 2004

Streptococcus canis was isolated from 31 milk samples from 11 cows in a dairy herd (with 49 lactating cows) affected by subclinical mastitis in north Rhine-Westphalia, Germany. Thirty-one isolates from the infected udder quarters were further characterized for their phenotypic and molecular properties. Most isolates (83.9%) produced {alpha}-galactosidase, and all were negative for ß-D-glucuronidase. Amplification of the 16S rRNA gene by the PCR method and digestion with the restriction enzymes RsaI, MspI, and AvaII yielded species-specific patterns. Additional identification by species-specific amplification of the 16S rRNA gene, the 16S-23S rRNA gene intergenic spacer region, the CAMP factor-encoding gene cfg, and the internal fragments of the sodA gene was consistent with S. canis. Macrorestriction analysis of the chromosomal DNA by pulsed-field gel electrophoresis showed that the S. canis isolates originated from a single clone or were very closely related.

* Corresponding author. Mailing address: Institut für Tierärztliche Nahrungsmittelkunde, Justus-Liebig-Universität Giessen, Ludwigstr. 21, D-35390 Giessen, Germany. Phone: 0049-641-9938952. Fax: 0049-641-9938959. E-mail: Abdulwahed.Hassan{at}vetmed.uni-giessen.de.

Journal of Clinical Microbiology, March 2005, p. 1234-1238, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1234-1238.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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