This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Neyra, E.
Right arrow Articles by Nolard, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Neyra, E.
Right arrow Articles by Nolard, N.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2005, p. 1348-1352, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1348-1352.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Epidemiology of Human Sporotrichosis Investigated by Amplified Fragment Length Polymorphism

Edgar Neyra,1 Pierre-Alain Fonteyne,2* Danielle Swinne,2,3 Frederic Fauche,2 Beatriz Bustamante,1 and Nicole Nolard2

Laboratorio de Micologia, Instituto de Medicina Tropical "Alexander von Humboldt," Universidad Peruana Cayetano Heredia, Lima, Peru,1 Mycology Section, Scientific Institute of Public Health, Brussels,2 Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium3

Received 13 August 2004/ Returned for modification 3 October 2004/ Accepted 18 November 2004

Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity of Peruvian strains of Sporothrix schenckii and to compare them to a panel of non-Peruvian strains. AFLP analysis suggests that the Peruvian strains can be divided into two homogeneous clusters with no reference to geographical origin or the clinical form of sporotrichosis. The strains from abroad present heterogeneous profiles, with the Bolivian strain and the Colombian strains related to one of the Peruvian population. Sequencing of internal transcribed spacer 2, used to examine the relationships over a longer distance, confirmed the division of Peruvian strains into two populations that can be identified on the basis of a single but specific sequence divergence. This paper introduces automated AFLP analysis as a valuable tool for further investigation of the epidemiology and ecology of S. schenckii.

* Corresponding author. Mailing address: Scientific Institute of Public Health, Mycology Section, Rue J. Wytsman 14, 1050 Brussels, Belgium. Phone: 32 (0) 26425533. Fax: 32 (0) 26425519. E-mail: PAFonteyne{at}iph.fgov.be.

Journal of Clinical Microbiology, March 2005, p. 1348-1352, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1348-1352.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pereira Silveira, C., Torres-Rodriguez, J. M., Alvarado-Ramirez, E., Murciano-Gonzalo, F., Dolande, M., Panizo, M., Reviakina, V. (2009). MICs and minimum fungicidal concentrations of amphotericin B, itraconazole, posaconazole and terbinafine in Sporothrix schenckii. J Med Microbiol 58: 1607-1610 [Abstract] [Full Text]  
  • de Meyer, E. M., de Beer, Z. W., Summerbell, R. C., Moharram, A.M., de Hoog, G. S., Vismer, H. F., Wingfield, M. J. (2008). Taxonomy and phylogeny of new wood- and soil-inhabiting Sporothrix species in the Ophiostoma stenoceras-Sporothrix schenckii complex. Mycologia 100: 647-661 [Abstract] [Full Text]  
  • Marimon, R., Cano, J., Gene, J., Sutton, D. A., Kawasaki, M., Guarro, J. (2007). Sporothrix brasiliensis, S. globosa, and S. mexicana, Three New Sporothrix Species of Clinical Interest. J. Clin. Microbiol. 45: 3198-3206 [Abstract] [Full Text]  
  • Marimon, R., Gene, J., Cano, J., Trilles, L., Dos Santos Lazera, M., Guarro, J. (2006). Molecular Phylogeny of Sporothrix schenckii.. J. Clin. Microbiol. 44: 3251-3256 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»