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Journal of Clinical Microbiology, March 2005, p. 1361-1365, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1361-1365.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Loss of the mecA Gene during Storage of Methicillin-Resistant Staphylococcus aureus Strains

Arjanne van Griethuysen,1* Inge van Loo,2 Alex van Belkum,3 Christina Vandenbroucke-Grauls,4 Wim Wannet,5 Peter van Keulen,2 and Jan Kluytmans2

Department of Medical Microbiology and Medical Immunology, Hospital Rijnstate, Arnhem,1 Department of Microbiology and Infection Control, Amphia Hospital, Breda,2 Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam,3 Department of Clinical Microbiology and Infection Control, VU Medical Center and Department of Medical Microbiology, Academic Medical Center, Amsterdam,4 National Institute of Public Health and the Environment, Bilthoven, The Netherlands5

Received 4 April 2004/ Returned for modification 5 August 2004/ Accepted 4 November 2004

The mecA gene was lost in 36 (14.4%) of 250 methicillin-resistant Staphylococcus aureus isolates after 2 years of storage at –80°C with the Microbank system (Pro-lab Diagnostics, Austin, Tex.). Further analysis of 35 of these isolates confirmed loss of the mecA gene in 32 isolates. This finding has important implications for the management of strain collections.

* Corresponding author. Mailing address: Department of Medical Microbiology and Medical Immunology, Internal Post Number 1630, Hospital Rijnstate Arnhem, P.O. Box 9025, 6800 EG Arnhem, The Netherlands. Phone: 31 26 3788880. Fax: 31 26 3787279. E-mail: AvanGriethuysen{at}alysis.nl.

Journal of Clinical Microbiology, March 2005, p. 1361-1365, Vol. 43, No. 3
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.3.1361-1365.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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