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Journal of Clinical Microbiology, April 2005, p. 1776-1781, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1776-1781.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Multilocus Sequence Typing versus Pulsed-Field Gel Electrophoresis for Characterization of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates

Lucia L. Nemoy,1,3* Mamuka Kotetishvili,1 Justine Tigno,1,4 Ananda Keefer-Norris,1 Anthony D. Harris,1,3 Eli N. Perencevich,1,3 Judith A. Johnson,2,3 Dave Torpey,1,3 Alexander Sulakvelidze,1 J. Glenn Morris Jr.,1 and O. Colin Stine1

Department of Epidemiology and Preventive Medicine,1 Department of Pathology, University of Maryland School of Medicine,2 VA Maryland Health Care System, Baltimore, Maryland,3 Department of Pathology, Case Western Reserve University, Cleveland, Ohio4

Received 28 September 2004/ Returned for modification 13 November 2004/ Accepted 20 December 2004

Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli strains are emerging pathogens. Molecular typing of ESBL-producing E. coli is useful for surveillance purposes, to monitor outbreaks and track nosocomial spread. Although pulsed-field gel electrophoresis (PFGE) is the current "gold standard" for bacterial molecular typing, multilocus sequence typing (MLST) may offer advantages. Forty ESBL-producing E. coli isolates were selected at random from a cohort of intensive care unit patients who had active surveillance perirectal cultures done. PFGE identified 19 unique PFGE types (PT) among the 40 isolates; MLST identified 22 unique sequence types. MLST had greater discriminatory ability than PFGE for ESBL-producing E. coli. Simpson's indices of diversity for PFGE and MLST were 0.895 and 0.956, respectively. There were five clonal complexes (CCs) (isolates with differences of no more than two loci) that each contained multiple PT, but each PT was found in only one CC, indicating genetic consistency within a CC. MLST has clear utility in studies of ESBL-producing E. coli, based on a greater discriminatory ability and reproducibility than PFGE and the ability to a priori define genetically related bacterial strains.

* Corresponding author. Mailing address: Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, MSTF Building, Room 9-34, 10 South Pine Street, Baltimore, MD 21201. Phone: (410) 706-0082. Fax: (410) 706-4581. E-mail: lnemoy{at}epi.umaryland.edu.

Journal of Clinical Microbiology, April 2005, p. 1776-1781, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1776-1781.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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