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Journal of Clinical Microbiology, April 2005, p. 1894-1901, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1894-1901.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Molecular Identification of Capnocytophaga spp. via 16S rRNA PCR-Restriction Fragment Length Polymorphism Analysis

Marilou Ciantar, Hubert N. Newman, Michael Wilson, and David A. Spratt*

Division of Microbial Diseases, Eastman Dental Institute, University College London, London, United Kingdom

Received 8 July 2004/ Returned for modification 29 August 2004/ Accepted 21 September 2004

Capnocytophaga spp. have been implicated as putative periodontal pathogens associated with various periodontal diseases. Although the genus is known to contain five human oral isolates, accurate identification to species level of these organisms recovered from subgingival plaque has been hampered by the lack of a reliable method. Hence, most studies to date have reported these isolates as Capnocytophaga spp. Previous attempts at identification were based on biochemical tests; however, the results were inconclusive. Considering the differing virulence features of the respective isolates, it is crucial to identify these isolates to species level. The universal and conservative nature of the 16S rRNA gene has provided an accurate method for bacterial identification. The aim of this study was to identify Capnocytophaga spp. via restriction enzyme analysis of this gene (16S rRNA PCR-restriction fragment length polymorphism). The results (backed up by 16S rRNA gene sequencing) showed that this method reliably identifies all named Capnocytophaga spp. to species level.

* Corresponding author. Mailing address: Division of Microbial Diseases, Eastman Dental Institute, UCL, 256 Gray's Inn Road, London WC1X 8LD, United Kingdom. Phone: 0044 20 7915 1107. Fax: 0044 20 7915 1127. E-mail: d.spratt{at}eastman.ucl.ac.uk.

Journal of Clinical Microbiology, April 2005, p. 1894-1901, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1894-1901.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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