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Journal of Clinical Microbiology, April 2005, p. 1910-1916, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1910-1916.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

env Gene Typing of Human Immunodeficiency Virus Type 1 Strains on Electronic Microarrays

N. A. Saunders,1* S. Alexander,2 and I. Tatt3,{dagger}

Genomics Proteomics Bioinformatics Unit,1 Sexually Transmitted Bacteria Laboratory,2 Sexually Transmitted and Blood-borne Virus Laboratory, Centre for Infections, London, United Kingdom3

Received 4 August 2004/ Returned for modification 8 October 2004/ Accepted 22 December 2004

The NanoChip system was used for subtyping human immunodeficiency virus type 1 (HIV-1) strains using probes complementary to the V1 region of the env gene. Probes for six subtypes (A to D, F, and G) and two circulating recombinant forms (AG and AE) of HIV-1 group M were included. The specificity of these oligonucleotides had been evaluated previously in a DNA enzyme immunoassay. Samples from 112 patient sera were used as templates in a nested reverse transcription-PCR to produce amplicons that were applied to the array. The array was then hybridized successively to pairs of oligonucleotide probes. The strains were assigned a subtype on the basis of their probe hybridization patterns. One strain gave a contradictory pattern and was designated as untypeable by the NanoChip assay. Eighty-eight strains gave hybridization patterns that allowed a correct subtype designation to be made by the NanoChip assay compared to either the sequence or the heteroduplex mobility assay (HMA)-determined subtypes. Thirteen strains that reacted with the subtype A probe (SA2) were incorrectly assigned to subtype A, or to one of the related circulating recombinant types (AE or AG), on the basis of reactions with probe SAE1 or SAG1. The results indicate that these oligonucleotides have relatively low specificities. The probe subtypes of three strains matched the subtypes determined for the gag and pol genes but not the env gene, suggesting that a recombination event may have occurred within the env gene. Overall, the NanoChip assay gave results comparable to those for HMA and sequencing and provides a convenient and cost-effective means by which to subtype HIV-1.

* Corresponding author. Mailing address: GPBU, Centre for Infections, HPA (Colindale), 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 44 20 8200 4400. Fax: 44 20 8358 3138. E-mail: nick.saunders{at}hpa.org.uk.

{dagger} Present address: WW Epidemiology (Neurosciences), GlaxoSmithKline Research and Development, Harlow, United Kingdom.

Journal of Clinical Microbiology, April 2005, p. 1910-1916, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1910-1916.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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