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Journal of Clinical Microbiology, April 2005, p. 1947-1949, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1947-1949.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Direct Identification of Major Blood Culture Pathogens, Including Pseudomonas aeruginosa and Escherichia coli, by a Panel of Fluorescence In Situ Hybridization Assays Using Peptide Nucleic Acid Probes

M. Søgaard,1* H. Stender,2 and H. C. Schønheyder1

Department of Clinical Microbiology, Aalborg Hospital-Aarhus University Hospital, Aalborg, Denmark ,1 AdvanDx Inc., Woburn, Massachusetts2

Received 20 August 2004/ Returned for modification 15 November 2004/ Accepted 14 December 2004

Rapid identification of four major pathogens from 1,231 positive blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes (AdvanDx Inc., Woburn, Mass.) was evaluated. For Escherichia coli, Staphylococcus aureus, and Candida albicans results agreed with conventional identification. The lower sensitivity of the Pseudomonas aeruginosa assay should not compromise the utility of the four assays.

* Corresponding author. Mailing address: Department of Clinical Microbiology, Aalborg Hospital Section South, DK-9000 Aalborg, Denmark. Phone: 45 99 32 32 19. Fax: 45 99 32 32 16. E-mail: aas.10915{at}nja.dk.

Journal of Clinical Microbiology, April 2005, p. 1947-1949, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1947-1949.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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