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Journal of Clinical Microbiology, April 2005, p. 1977-1978, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1977-1978.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of a Rapid Assay as an Alternative to Conventional Enzyme Immunoassays for Detection of Hepatitis C Virus-Specific Antibodies

Hubert Darius J. Daniel, Priya Abraham,* Sukanya Raghuraman, Perumal Vivekanandan, Thenmozhi Subramaniam, and Gopalan Sridharan

Department of Clinical Virology, Christian Medical College, Vellore, India

Received 13 August 2004/ Returned for modification 9 November 2004/ Accepted 29 November 2004

A rapid membrane flow-through immunoassay to detect antibodies to hepatitis C virus was compared with a commercial enzyme immunoassay (EIA) and microparticle enzyme immunoassay (MEIA) using 2,590 serum samples. Sensitivity and specificity of the "rapid assay" in comparison to the EIA/MEIA were 99.3 and 99.0%; the correlation coefficient being 0.91. This assay is suitable where infrastructure and laboratory expertise are limited.

* Corresponding author. Mailing address: Department of Clinical Virology, Christian Medical College, Vellore 632004, India. Phone: 91-416-2222102, ext. 2772. Fax: 91-416-2232035. E-mail: priyaabraham{at}cmcvellore.ac.in.

Journal of Clinical Microbiology, April 2005, p. 1977-1978, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1977-1978.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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