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Journal of Clinical Microbiology, April 2005, p. 1982-1984, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1982-1984.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Development and Application of a Saccharomyces cerevisiae-Expressed Nucleocapsid Protein-Based Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against Infectious Bronchitis Virus

Aliandra M. Gibertoni,1,3 Maria de Fátima S. Montassier,1 Janete A. D. Sena,2 Patrícia E. N. Givisiez,1 Cibele R. A. G. Furuyama,1 and Hélio J. Montassier1*

Laboratório de Virologia e Imunologia, Departamento de Patologia Veterinária,1 Laboratório de Genética de Bactérias, Departamento de Biologia Aplicada à Agropecuária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, 14884-900 Jaboticabal,2 Fundação de Amparo à Pesquisa do Estado de São Paulo, Sao Paulo, Brazil3

Received 9 July 2004/ Returned for modification 13 September 2004/ Accepted 16 December 2004

A Saccharomyces cerevisiae-expressed nucleocapsid (N) polypeptide of the M41 strain of infectious bronchitis virus (IBV) was used as antigen in a recombinant yeast-expressed N protein-based enzyme-linked immunosorbent assay (Y-N-ELISA). The Y-N-ELISA was rapid, sensitive, and specific for detecting chicken serum antibodies to IBV, and it compared favorably with a commercial ELISA.

* Corresponding author. Mailing address: Laboratório de Virologia e Imunologia, Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, 14884-900, Jaboticabal, Sao Paulo, Brazil. Phone: 55-16-3209-26-52. Fax: 55-16-3202-42-75. E-mail: heliojm{at}fcav.unesp.br.

Journal of Clinical Microbiology, April 2005, p. 1982-1984, Vol. 43, No. 4
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.4.1982-1984.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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