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Journal of Clinical Microbiology, May 2005, p. 2330-2338, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2330-2338.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Genomic Diversity in Campylobacter jejuni: Identification of C. jejuni 81-176-Specific Genes

Frédéric Poly,1,2 Deborah Threadgill,3 and Alain Stintzi1*

Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, Oklahoma 74078,1 Dynamique, Evolution et Expression de Genomes de Microorganismes, Université Louis Pasteur/CNRS FRE 2326, 67000 Strasbourg, France,2 Department of Genetics, University of North Carolina, Chapel Hill, North Carolina 27599-72643

Received 3 September 2004/ Returned for modification 14 December 2004/ Accepted 27 December 2004

Since the publication of the complete genomic sequence of Campylobacter jejuni NCTC 11168 in February 2000, evidence has been compiling that suggests C. jejuni strains exhibit high genomic diversity. In order to investigate this diversity, the unique genomic DNA sequences from a nonsequenced Campylobacter strain, C. jejuni 81-176, were identified by comparison with C. jejuni NCTC 11168 by using a shotgun DNA microarray approach. Up to 63 kb of new chromosomal DNA sequences unique to this pathogen were obtained. Eighty-six open reading frames were identified by the presence of uninterrupted coding regions encoding a minimum of 40 amino acids. In addition, this study shows that the whole-plasmid shotgun microarray approach is effective and provides a comprehensive coverage of DNA regions that differ between two closely related genomes. The two plasmids harbored by this Campylobacter strain, pTet and pVir, were also sequenced, with coverages of 2.5- and 2.9-fold, respectively, representing 72 and 92% of their complete nucleotide sequences. The unique chromosomal genes encode proteins involved in capsule and lipooligosaccharide biosynthesis, restriction and modification systems, and respiratory metabolism. Several of these unique genes are likely associated with C. jejuni 81-176 fitness and virulence. Interestingly, the comparison of C. jejuni 81-176 unique genes with those of C. jejuni ATCC 43431 revealed a single gene which encodes a probable TraG-like protein. The product of this gene might be associated with the mechanism of C. jejuni invasion into epithelial cells. In conclusion, this study extends the repertoire of C. jejuni genes and thus will permit the construction of a composite and more comprehensive microarray of C. jejuni.

* Corresponding author. Mailing address: Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK 74078. Phone: (405) 744-4518. Fax: (405) 744-5275. E-mail: stintzi{at}cvm.okstate.edu.

Journal of Clinical Microbiology, May 2005, p. 2330-2338, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2330-2338.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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