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Journal of Clinical Microbiology, May 2005, p. 2449-2451, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2449-2451.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Use of Monoclonal Antibodies To Serotype Bordetella pertussis Isolates: Comparison of Results Obtained by Indirect Whole-Cell Enzyme-Linked Immunosorbent Assay and Bacterial Microagglutination Methods

Raymond S. W. Tsang,1* Michelle L. Sill,1 Abdolreza Advani,2 Dorothy Xing,3 Penny Newland,3 and Hans Hallander2

Vaccine Preventable Bacterial Diseases Division, National Microbiology Laboratory, Population and Public Health Branch, Health Canada, Winnipeg, Manitoba, Canada,1 Swedish Institute for Infectious Disease Control, Solna, Sweden,2 Division of Bacteriology, National Institute for Biological Standards and Controls, Blanche Lane, Potters Bar, United Kingdom3

Received 26 November 2004/ Returned for modification 4 January 2005/ Accepted 6 January 2005

Sixty-one Bordetella pertussis isolates were tested blindly in two laboratories to determine their serotype nature by monoclonal antibodies using two independent methods: the standard bacterial microagglutination assay and an indirect whole-cell enzyme-linked immunosorbent assay. Both methods gave concordant results in 60 of the 61 isolates.

* Corresponding author. Mailing address: Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2. Phone: (204) 789-6020. Fax: (204) 789-2018. E-mail: rtsang{at}hc-sc.gc.ca.

Journal of Clinical Microbiology, May 2005, p. 2449-2451, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2449-2451.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tsang, R. S. W. (2008). Serotyping and Population Genetics of Invasive Haemophilus influenzae. J. Clin. Microbiol. 46: 1159-1159 [Full Text]  


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