This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Watanabe, H.
Right arrow Articles by Oishi, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Watanabe, H.
Right arrow Articles by Oishi, K.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2005, p. 2474-2476, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2474-2476.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Comparative Molecular Analysis of Haemophilus influenzae Isolates from Young Children with Acute Lower Respiratory Tract Infections and Meningitis in Hanoi, Vietnam

Hiroshi Watanabe,1* Chiharu Kaji,1 Dang Duc Anh,2 Phan Le Thanh Huong,2 Nguyen Thi Hien Anh,2 Vu Thi Huong,2 Hoang Vu Mai Phuong,2 Ngo Thi Thi,3 Pham Thi Suu,3 Nguyen Thi Thu Nguyet,3 Olivia Sebastian Rusizoka,1 Kiwao Watanabe,1 Tsuyoshi Nagatake,1 and Kazunori Oishi1

Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan,1 National Institute of Hygiene and Epidemiology,2 National Institute of Pediatrics, Hanoi, Vietnam3

Received 10 December 2004/ Accepted 11 December 2004

Thirty-seven Haemophilus influenzae strains from nasopharyngeal swabs (NP) and 44 H. influenzae strains from cerebrospinal fluid (CSF) were investigated. Of the 37 H. influenzae isolates from NP, the serotypes of 30 isolates were nontypeable, 4 were type b, 2 were type c, and 1 was type a, whereas all of the 44 isolates from CSF were type b. The MICs of 16 antibiotics for the H. influenzae isolates from NP and CSF were similar, and no ß-lactamase-negative ampicillin-resistant strain was found. Molecular typing by pulsed-field gel electrophoresis (PFGE) showed that the 37 H. influenzae strains from NP had 22 PFGE patterns, with none predominating, and the 44 H. influenzae strains from CSF had 9 PFGE patterns, with patterns {alpha} (22 isolates) and ß (12 isolates) predominating. Our results indicate that two predominant types of H. influenzae type b strains have the potential to spread among children with meningitis in Hanoi, Vietnam.

* Corresponding author. Mailing address: Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan. Phone: 81 (95) 849-7842. Fax: 81 (95) 849-7843. E-mail: h-wata{at}net.nagasaki-u.ac.jp.

Journal of Clinical Microbiology, May 2005, p. 2474-2476, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2474-2476.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»