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Journal of Clinical Microbiology, May 2005, p. 2489-2491, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2489-2491.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Isothermal RNA Sequence Amplification Method for Rapid Antituberculosis Drug Susceptibility Testing of Mycobacterium tuberculosis

Shunji Takakura,1* Shigeo Tsuchiya,2 Naoko Fujihara,1 Toyoichiro Kudo,1 Yoshitsugu Iinuma,1 Satoshi Mitarai,3 Satoshi Ichiyama,1 Kiyoshi Yasukawa,2 and Takahiko Ishiguro2

Department of Clinically Laboratory Medicine, Graduate School of Medicine, Kyoto University, 54 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 6068507, Japan,1 Scientific Instruments Division, Tokyo Research Laboratories, Tosoh Corporation, 2743-1 Hayakawa, Ayase-shi, Kanagawa 2521123, Japan,2 Bacteriology Division, The Reference Centre for Mycobacterium, The Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo 2048533, Japan3

Received 19 November 2003/ Accepted 3 January 2005

RNA transcript quantification by an isothermal sequence amplification reaction was evaluated for susceptibility testing of 15 Mycobacterium tuberculosis strains. Agreement with the proportion method on Ogawa egg medium and the BACTEC MGIT 960 system was 100 and 87% for rifampin, 93 and 100% for isoniazid, 60 and 53% for ethambutol, and 80 and 80% for streptomycin, respectively.

* Corresponding author. Mailing address: Department of Clinical Laboratory Medicine, Graduate School of Medicine, Kyoto University, 54 Shogoin-Kawaharacho, Sakyo-ku, Kyoto, 606-8507, Japan. Phone: 81-75-751-4914. Fax: 81-75-751-3233. E-mail: stakakr{at}kuhp.kyoto-u.ac.jp.

Journal of Clinical Microbiology, May 2005, p. 2489-2491, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2489-2491.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Somoskovi, A., Clobridge, A., Larsen, S. C., Sinyavskiy, O., Surucuoglu, S., Parsons, L. M., Salfinger, M. (2006). Does the MGIT 960 System Improve the Turnaround Times for Growth Detection and Susceptibility Testing of the Mycobacterium tuberculosis Complex?. J. Clin. Microbiol. 44: 2314-2315 [Full Text]  
  • Takakura, S., Tsuchiya, S., Isawa, Y., Yasukawa, K., Hayashi, T., Tomita, M., Suzuki, K., Hasegawa, T., Tagami, T., Kurashima, A., Ichiyama, S. (2005). Rapid Detection of Mycobacterium tuberculosis in Respiratory Samples by Transcription-Reverse Transcription Concerted Reaction with an Automated System. J. Clin. Microbiol. 43: 5435-5439 [Abstract] [Full Text]  


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