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Journal of Clinical Microbiology, May 2005, p. 2500-2502, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2500-2502.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Distribution of IS1311 and IS1245 in Mycobacterium avium Subspecies Revisited

Tone Bjordal Johansen, Berit Djønne, Merete R. Jensen, and Ingrid Olsen*

Department of Animal Health, National Veterinary Institute, Oslo, Norway

Received 7 October 2004/ Returned for modification 28 November 2004/ Accepted 14 January 2005

We demonstrated that IS1245 is not present in Mycobacterium avium subsp. paratuberculosis by restriction fragment length polymorphism and that the designated three-banded bird pattern of IS1245 in M. avium subsp. avium consists of one copy of IS1245 and two copies of IS1311. Cross hybridization between the two elements can be avoided by using more specific probes.

* Corresponding author. Mailing address: National Veterinary Institute, Pb 8156 Dep., 0033 Oslo, Norway. Phone: 47 23 21 63 32. Fax: 47 23 21 63 02. E-mail: Ingrid.Olsen{at}vetinst.no.

Journal of Clinical Microbiology, May 2005, p. 2500-2502, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2500-2502.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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