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Journal of Clinical Microbiology, May 2005, p. 2523-2525, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2523-2525.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Methods for Reducing Bacterial Contamination in the BacT/Alert Mycobacterial Culture Detection System

Kim Dionne, Amy Sweeney, Annie Hedgepeth, Karen Carroll, and Nicole Parrish*

Division of Medical Microbiology, The Johns Hopkins Medical Institutions, Meyer B1-193, 600 N. Wolfe St., Baltimore, Maryland 21287

Received 24 September 2004/ Returned for modification 3 November 2004/ Accepted 23 December 2004

Two methods were compared for decreasing bacterial contamination in the BacT/Alert mycobacterial culture detection system. Two concentrations, 0.5 ml (standard amount) and 1.0 ml, of mycobacterial antibiotic supplement were evaluated. Contamination rates were 14% and 6% for the standard and the doubled concentrations, respectively. This difference was statistically significant (P < 0.005).

* Corresponding author. Mailing address: Johns Hopkins University, Meyer B1-193, 600 N. Wolfe Street, Baltimore, MD 21287. Phone: (410) 550-3525. Fax: (410) 558-9098. E-mail: nparrish{at}jhmi.edu.

Journal of Clinical Microbiology, May 2005, p. 2523-2525, Vol. 43, No. 5
0095-1137/05/$08.00+0     doi:10.1128/JCM.43.5.2523-2525.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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